Table 4.
Primers used for the detection of virulence genes.
| Target Gene | Primer Sequence (5′–3′) | Product Size (bp) | Annealing Temperature (°C) | References |
|---|---|---|---|---|
| cadF | F-TTGAAGGTAATTTAGATATG R-CTAATACCTAAAGTTGAAAC |
400 | 43 | [41] |
| ciaB | F-TGCGAGATTTTTCGAGAATG R-TGCCCGCCTTAGAACTTACA |
527 | 54 | [41] |
| dnaJ | F-ATTGATTTTGCTGCGGGTAG R-ATCCGCAAAAGCTTCAAAAA |
177 | 50 | [41] |
| pldA | F-AAGAGTGAGGCGAAATTCCA R-GCAAGATGGCAGGATTATCA |
385 | 46 | [41] |
| cdtA | F-CCTTGTGATGCAAGCAATC R-ACACTCCATTTGCTTTCTG |
370 | 49 | [21] |
| cdtB | F-GTTAAAATCCCCTGCTATCAACCA R-GTTGGCACTTGGAATTTGCAAGGC |
495 | 51 | |
| cdtC | F-CGATGAGTTAAAACAAAAAGATA R-TTGGCATTATAGAAAATACAGTT |
182 | 48 |