Table 6.

Cycling conditions for PCR

Gene		Initial Denaturation	Denaturation	Annealing	Extension	Number of Cycles	Final Extension
Species-specific	phoA	94 °C 5 min	94 °C 30 s	58 °C 45 s	72 °C 45 s	35	72 °C 10 min
Virulence	Iss	94 °C 5 min	94 °C 30 s	58 °C 45 s	72 °C 45 s	35	72 °C 10 min
	fimH	95 °C 2 min	94 °C 30 s	58 °C 30 s	72 °C 1 min	33	72 °C 7 min
	iutA	94 °C 3 min	94 °C 1 min	55 °C 1 min	72 °C 30 s	30	72 °C 7 min