Table 1.
Differentially expressed proteins identified using SWATH (Sequential Window Acquisition of All Theoretical Mass Spectra) MS (mass spectrometry) analysis of MDA-MB-468 cell lysate and secretome following adrenoceptor agonism/antagonism.
| Lysate | Secretome | |||||
|---|---|---|---|---|---|---|
| Treatment (vs UT) | Protein | Swiss-Prot ID | Log2 Fold Change | Protein | SwissProt ID | Log2 Fold Change |
| NE | LYPD3 | O95274 | 2.235 | IF5A1 | P63241 | 2.181 |
| CRYAB | P02511 | 1.342 | H2BFS | P57053 | 2.066 | |
| NDRG1 | Q92597 | 1.021 | LYPD3 | O95274 | 1.577 | |
| CASPE | P31944 | 1.015 | H4 | P62805 | 1.489 | |
| AATM | P10809 | −1.010 | STC1 | P52823 | 1.077 | |
| CH60 | P10809 | −1.141 | LIF | P15018 | −2.090 | |
| HINT2 | Q9BX68 | −1.206 | ||||
| MDHM | P40926 | −1.315 | ||||
| ECH1 | Q13011 | −1.431 | ||||
| H14 | P10412 | −1.515 | ||||
| ODO4 | P36957 | −1.520 | ||||
| H12 | P16403 | −1.574 | ||||
| ISO | IF172 | Q9UG01 | 2.684 | DPY30 | Q9C005 | 1.320 |
| LYPD3 | O95274 | 2.564 | ADML | P35318 | 1.142 | |
| CRYAB | P02511 | 1.413 | H4 | P62805 | 1.078 | |
| ANXA1 | P04083 | 1.065 | ||||
| CTGF | P29279 | −1.151 | ||||
| UBA3 | Q8TBC4 | −1.995 | ||||
| LAMC1 | P11047 | −3.499 | ||||
| ICI 118,551 | S10-A7 | P31151 | 1.271 | NDKA | P15531 | 3.784 |
| IF5A1 | P63241 | 2.252 | ||||
| S10A6 | P06703 | 1.651 | ||||
| ANXA2 | P07355 | 1.311 | ||||
| DPY30 | Q9C005 | 1.286 | ||||
| EDF1 | O60869 | −2.432 | ||||
| ICI 118,551 + NE | KRT36 | O76013 | 2.160 | CRK | P46108 | 2.591 |
| CRYAB | P62750 | 1.776 | NUCL | P19338 | 1.947 | |
| NDRG1 | P02768 | −1.067 | HS90A | P07900 | 1.887 | |
| APOA1 | P02647 | 1.769 | ||||
| ANXA2 | P07355 | 1.694 | ||||
| CUTA | O60888 | 1.642 | ||||
| STC1 | P52823 | 1.474 | ||||
| FETUA | P02765 | 1.418 | ||||
| LYPD3 | O95274 | 1.308 | ||||
| H4 | P62805 | 1.140 | ||||
| S100P | P25815 | 1.139 | ||||
| SFRP1 | Q8N474 | 1.136 | ||||
| TSP1 | P07996 | −1.280 | ||||
| EDF1 | O60869 | −4.362 | ||||
| BSSP4 | Q0GZN4 | −4.870 | ||||
Log2 fold change represents the increase/decrease in protein expression relative to untreated samples (UT). Proteins with a Log2 fold change of <1.0 or >1.0 and OneOmics confidence of ≥60% are shown. Proteins with single peptides are excluded. Data is from six biological replicates. NE—norepinephrine, ISO—isoproterenol, UT—untreated.