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. 2020 Feb 7;8(2):26. doi: 10.3390/biomedicines8020026

Figure 2.

Figure 2

Validation of glucose concentration in four perifusion protocols. (A) Single pulse of glucose (filled line; HG = 28 mM) and potassium protocol (dotted line; KCl = 50 mM) SPGP. (B) Double pulse of high glucose (HG = 17 mM) protocol (DPG). (C) Ascending ramp of glucose concentration from 3–30 mM in 130 min (ARG). (D) Constant high-glucose concentration (HG = 17 mM) protocol (CHG). Data are expressed as mean ± SD of n = 4 independent experiments. Arrows indicate the direction of transition between stimuli.