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RT-PCR amplification and Sanger sequencing of WT and mutant splicing reporter minigenes using vector-specific primers. The results of RT-PCR analysis using vector specific primers are shown along with a diagram of the transcripts obtained in Hep3B cells after 24 h of incubation (a) and their sequencing results depicted in electropherograms (b). c.1213+5G>T [IVS6+5G>T]: mutant minigene; WT: wild-type; NT: non-transfected.
