Table 5.
Primers used to amplify the following P. vivax gene targets: the multidrug resistance 1 (Pvmdr1) gene, the putative transporter protein (Pvcrt-o) gene, the dihydrofolate reductase (Pvdhfr) gene and the dihydropteroate synthetase (Pvdhps) gene.
| Name | Sequences 5’–3’ | Gene Targets | Product Size | Tm. (°C) | References |
|---|---|---|---|---|---|
| Pvdhfr (outer) F Pvdhfr (outer) R |
CACCGCACCAGTTGATTCCT CCTCGGCGTTGTTCTTCT |
Pvdhfr | 979 | 67.3 63.0 |
[25] |
| Pvdhfr (nested) F Pvdhfr (nested) R |
CCCCACCACATAACGAAG CCCCACCTTGCTGTAAACC |
Pvdhfr | 755 | 61.5 64.2 |
[25] |
| Pvdhps (outer) F Pvdhps (outer) R |
GATGGCGGTTTATTTGTCG GCTGATCTTTGTCTTGACG |
Pvdhps | 1009 | 62.8 58.5 |
[25] |
| Pvdhps (nested) F Pvdhps (nested) R |
GCTGTGGAGAGGATGTTC CCGCTCATCAGTCTGCAC |
Pvdhps | 731 | 58.2 63.5 |
[25] |
| Pvcrt-o F Pvcrt-o R |
CAGTGAGAAGCCCCTGTTCG CCGCTCATCAGTCTGCAC |
Pvcrt-o | 750 | 67.2 68.5 |
* |
| Pvmdr F Pvmdr R |
GCGAACTCGAATAAGTACTCCCTCTA GGCGTAGCTTCC CGTAAATAAA |
Pvmdr1 | 762 | 65.4 64.8 |
[4] |
* A new primer set was designed by the authors to detect mutations in the resistant marker. (Tm = annealing temperature).