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. 2020 Feb 19;9(2):92. doi: 10.3390/antibiotics9020092

Figure 1.

Figure 1

Fluorescence excitation (A) and emission (B) spectra of bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC4(3)) + Propidium Iodide (PI) and fluorescence excitation (C,E) and emission (D,F) spectra of 3,3′-dipropylthiadicarbocyanine iodide (diSC3(5)) +/− PI. (A) Excitation spectra with 516 nm detection wavelength (DiBAC4(3) emission maximum); (B) emission spectra with 496 nm excitation wavelength (DiBAC4(3) excitation maximum) of three different DiBAC4(3) concentrations + 10 µg/mL PI; (C,E) excitation spectra with 672 nm (diSC3(5) emission maximum) and 617 nm (PI emission maximum) detection wavelengths, respectively, and (D,F) emission spectra with 652 nm (diSC3(5) excitation maximum) and 490 nm (PI excitation maximum) excitation wavelengths, respectively, of 400 nM diSC3(5) + 20, 10, and 5 µg/mL PI. For technical reasons due to instrument settings, spectra in (A–D) could not be measured at wavelengths >496 nm, <510 nm, >650 nm, and <670 nm, respectively. In (A), (C), and (D) error bars were omitted for clarity.