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Interference of uncouplers and peptides with the fluorescent dyes. The kinetics of (A) diSC3(5) (λex = 652 nm, λem = 672 nm) and (B) PI (λex = 535 nm, λem = 617 nm) are shown in separate graphs for clarity purposes. The time necessary for the addition of peptides and uncouplers (indicated by *) was about 6 min.
