Figure 2.

(A) Representative scheme of the different steps applied for the preparation of crude protein extracts from the yellows berries of L. europaeus. a: yellow berries; b: pitted berries; c: powdered berries in liquid nitrogen using a mortar and pestle; d: centrifugation of the mixture; e: crude protein extract. Finely ground powder of plant fruit was used as starting material in all three protocols. (B) Table reporting protein yields from berries of L. europaeus using three extraction protocols. Data are presented as means ± standard deviation (s.d.) of three different samples analyzed in triplicate.