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SDS-PAGE (10%) analysis of the total protein extracts from L. europaeus berries using the three methods. Lane 1: molecular weight markers (Thermo Scientific); crude protein extracts obtained by: method 1 (Lane 2); method 2 (Lane 3); and method 3 (Lane 4). Protein bands were detected by Coomassie blue staining. Equal amounts of proteins were loaded for each Lane. The gel is representative of three independent experiments on three different protein preparations.
