Table 7.
Overview of the antibiotics screened for in the pig manure.
| Antbiotic Residue | Precurson ion (m/z) | Product ion (m/z) | RT a (min) | Antbiotic Residue | Precurson ion (m/z) | Product ion (m/z) | RT a (min) | ||
|---|---|---|---|---|---|---|---|---|---|
| β-lactam antibiotics | Quinolones | ||||||||
| amoxicillin | 366.01 | > | 207.93 | 1 | cinoxacin | 262.90 | > | 188.99 | 4.31 |
| ampicillin | 349.95 | > | 106.02 | 2.9 | nalidixic acid | 232.86 | > | 130.93 | 4.95 |
| benzylpenicillin | 334.94 | > | 175.95 | 4.79 | oxolinic acid | 261.90 | > | 159.91 | 4.59 |
| cefalexin | 347.93 | > | 157.97 | 2.99 | |||||
| cefalonium | 458.98 | > | 336.91 | 2.78 | Fluoroquinolones | ||||
| cefapirin | 423.90 | > | 291.94 | 1.47 | ciprofloxacin | 331.97 | > | 230.96 | 3.55 |
| +metabolite desacetyl cefapirin | 381.89 | > | 111.86 | 0.82 | danofloxacin | 357.99 | > | 82.02 | 3.71 |
| cefazolin | 454.87 | > | 322.93 | 3.49 | difloxacin | 400.04 | > | 298.97 | 4.15 |
| cefoperazone | 668.40 | > | 165.00 | 5.08 | enoxacin | 321.03 | > | 205.67 | 3.34 |
| cefquinome | 529.03 | > | 134.02 | 2.86 | enrofloxacin | 360.00 | > | 245.01 | 3.8 |
| ceftiofur | 523.97 | > | 240.90 | 4.95 | norfloxacin | 319.97 | > | 232.99 | 3.46 |
| +metabolite desfuroylceftiofur cystine disulfide | 523.97 | > | 240.90 | 4.59 | flumequine | 261.98 | > | 201.99 | 5.01 |
| cloxacillin | 435.91 | > | 276.91 | 5.12 | marbofloxacin | 362.98 | > | 72.07 | 3.29 |
| dicloxacillin | 469.99 | > | 160.11 | 5.26 | sarafloxacin | 385.96 | > | 299.01 | 4.1 |
| nafcillin | 414.97 | > | 198.97 | 5.18 | |||||
| oxacillin | 402.01 | > | 242.93 | 5.03 | Lincosamides | ||||
| penicillin V | 350.97 | > | 160.02 | 4.93 | lincomycin | 407.20 | > | 126.20 | 2.4 |
| pirlimycin | 411.07 | > | 363.05 | 3.99 | |||||
| Tetracyclines+epimers | Sulfonamides and | ||||||||
| chlortetracycline | 479.02 | > | 443.93 | 4.35 | trimethoprim | ||||
| doxycycline | 444.99 | > | 320.98 | 4.41 | sulfachloropyridazine | 284.85 | > | 92.03 | 3.99 |
| oxytetracycline | 460.99 | > | 425.97 | 3.32 | sulfaclozine | 284.92 | > | 92.03 | 3.99 |
| tetracycline | 444.99 | > | 409.98 | 3.6 | sulfadiazine | 250.89 | > | 92.03 | 1.79 |
| sulfadimethoxine | 310.91 | > | 155.94 | 4.73 | |||||
| Macrolides | sulfadoxine | 310.91 | > | 92.03 | 4.23 | ||||
| erythromycin A | 734.43 | > | 158.00 | 4.79 | sulfamerazine | 264.91 | > | 107.97 | 2.65 |
| gamithromycin | 777.52 | > | 619.33 | 4.58 | sulfamethazine | 278.92 | > | 92.03 | 3.3 |
| spiramycin | 843.42 | > | 174.06 | 4.38 | sulfamethoxazole | 253.89 | > | 92.03 | 4.25 |
| tilmicosin | 869.47 | > | 174.00 | 4.65 | sulfamethoxypyridazine | 280.97 | > | 92.04 | 3.49 |
| tulathromycin | 806.61 | > | 577.21 | 3.92 | sulfapyridine | 249.90 | > | 92.04 | 2.33 |
| tylosin A | 916.58 | > | 174.05 | 4.85 | sulfaquinoxaline | 300.91 | > | 92.03 | 4.74 |
| tylvalosin | 1042.37 | > | 174.09 | 5.32 | sulfathiazole | 255.85 | > | 108.03 | 2.25 |
| trimethoprim | 290.98 | > | 230.01 | 3.19 | |||||
| Pleuromutilines | |||||||||
| tiamulin | 494.23 | > | 192.02 | 5.03 | Amphenicols | ||||
| valnemulin | 565.26 | > | 263.06 | 5.09 | chloramphenicol | 320.85 | > | 151.85 | 4.52 |
| florfenicol | 355.78 | > | 184.87 | 4.26 | |||||
| Aminoglycosides | thiamphenicol | 353.85 | > | 289.89 | 3.11 | ||||
| apramycin | 540.12 | > | 199.03 | 6.79 | Diaminopyrimidine | ||||
| 540.12 | > | 216.98 | derivates | ||||||
| 540.12 | > | 378.00 | dapsone | 248.90 | > | 92.03 | 3.97 | ||
| dihydrostreptomycin | 292.86 | > | 86.17 | 5.83 | |||||
| 292.86 | > | 176.12 | Polymyxins | ||||||
| 292.86 | > | 263.07 | colistin B | 578.59 | > | 101.46 | 6.54 | ||
| gentamicin | 464.35 | > | 322.23 | 6.87 | 578.59 | > | 227.92 | ||
| (sum of C1, C1a, C2 and C2a) | 478.25 | > | 322.23 | 578.59 | > | 570.20 | |||
| 450.25 | > | 322.25 | |||||||
| kanamycin A | 485.10 | > | 163.20 | 6.57 | colistin A | 585.56 | > | 101.46 | 6.51 |
| 485.10 | > | 187.20 | 585.56 | > | 202.09 | ||||
| 485.10 | > | 324.20 | 585.56 | > | 576.86 | ||||
| neomycin B | 615.21 | > | 161.14 | 6.98 | Internal standards | ||||
| 615.21 | > | 163.27 | cefotaxime | 455.90 | > | 124.99 | 3.22 | ||
| 616.42 | > | 203.23 | ceftiofur-d3 | 527.06 | > | 244.09 | 4.59 | ||
| 615.21 | > | 293.07 | chloramphenicol-d5 | 325.85 | > | 156.85 | 4.5 | ||
| 615.21 | > | 323.19 | cincophen | 249.92 | > | 127.98 | 5.06 | ||
| paromomycin | 616.42 | > | 163.03 | 6.79 | clindamycin | 425.08 | > | 126.10 | 4.44 |
| 616.42 | > | 293.26 | lomefloxacine | 351.98 | > | 265.02 | 3.68 | ||
| 616.42 | > | 324.39 | methacycline | 443.10 | > | 381.09 | 4.4 | ||
| spectinomycin | 350.94 | > | 98.05 | 3.1 | piperacillin | 540.00 | > | 181.94 | 4.71 |
| 350.94 | > | 315.00 | polymyxin B1 | 602.54 | > | 101.03 | 4.34 | ||
| 350.94 | > | 332.91 | ribostamycin | 455.37 | > | 295.23 | 6.51 | ||
| streptomycin | 291.50 | > | 176.01 | 5.8 | roxithromycin | 837.37 | > | 158.01 | 5.07 |
| 291.50 | > | 263.21 | sulfadimethoxine 13C6 | 316.96 | > | 98.08 | 4.73 | ||
| 291.50 | > | 295.23 | trimethoprim-d9 | 300.04 | > | 263.98 | 3.12 | ||
| tobramycin | 468.10 | > | 162.96 | 6.9 | |||||
| 468.10 | > | 324.05 | |||||||
| 468.10 | > | 144.95 | |||||||
a: RT: Retention time. For the multi-residue method (all residues except aminoglycosides and polymyxins), a screening method was used, where the transition was followed from the precursor ion to one product ion. In case a signal was obtained, the sample was reinjected and minimum 2 product ions were followed.