Table 9.
Overview of the used concentrations during the validation, limit of detection (LOD), limit of quantification (LOQ), intralaboratory reproducibility (RSDR), expanded measurement uncertainty (U), mean linearity (R2) for the selected aminoglycosides in pig manure at the different validation levels.
| Antibiotic Residue | Spiked Levels for Validation (µg/kg) | LOD (µg/kg) | LOQ (µg/kg) | Repeatability (%) | RSDR (%) | U (%) | Linearity (R2) |
|---|---|---|---|---|---|---|---|
| apramycin | 250, 500, 1000 | 34 | 113 | 8 | 9 | 29 | 0.9946 |
| dihydrostreptomycin | 250, 500, 1000 | 10 | 33 | 6 | 8 | 22 | 0.9938 |
| gentamicin | 250, 500, 1000 | 21 | 72 | 9 | 8 | 31 | 0.9899 |
| kanamycin | 250, 500, 1000 | 73 | 243 | 20 | 26 | 64 | 0.9763 |
| neomycin | 250, 500, 1000 | 47 | 157 | 11 | 15 | 46 | 0.9885 |
| paromomycin | 250, 500, 1000 | 29 | 97 | 6 | 9 | 30 | 0.9954 |
| spectinomycin | 1000, 2000, 4000 | 394 | 1313 | 25 | 55 | 126 | 0.9112 |
| streptomycin | 500, 1000, 2000 | 32 | 107 | 7 | 7 | 22 | 0.9946 |
| tobramycin | 250, 500, 1000 | 17 | 57 | 8 | 10 | 35 | 0.9895 |