Figure 3.

ELISA assay demonstrating aggrecan adsorption to wells. No aggrecan or aggrecan at 150 or 300 µg/mL was plated into wells of a 96-well plate and were subjected to the enzyme digestion protocol. Wells were reacted with primary antibody against the G1-domain, reacted with a horseradish peroxidase secondary antibody, incubated with peroxidase substrate, and the absorbance was read at 450 nm. ANOVA analysis was conducted followed by a Bonferroni post-hoc analysis. Asterisks (*) represent differences in absorbance from the no aggrecan condition while Δ represents differences from 150 µg/mL intact aggrecan. *** p < 0.001, Δ p < 0.05, ΔΔ p < 0.01, ΔΔΔ p < 0.001.