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. 2020 Feb 18;8(2):35. doi: 10.3390/biomedicines8020035

Figure 5.

Figure 5

The compound OI causes a significant increase in ROS levels. Both MCF-7 (a) and HeLa (b) cells were treated for 24 h with 15 and 30 µM OI, respectively, alone and/or in the presence of N-acetyl-l-cysteine (NAC); ROS levels were evaluated by FACS analysis using the CM-H2DCFDA probe. Cell proliferation assay (MTT assay) was performed in both treated MCF-7 (c) and HeLa (d) cells, in the presence or absence of 3 mM NAC. Note that after 72 h, in the presence of NAC, cells displayed recovery in growth. Values represent means ± SD of three different experiments, each performed with triplicate samples. * p value < 0.05; **** p value < 0.0001.