Figure 5.

PCR‐MS approach‐TIGER: Aliquots of nucleic acids present in a sample are deposited into wells of a micro‐titer plate to begin PCR. Each well contains a pair of broad‐range primers that target a selected domain of microorganisms. PCR products are desalted and electrosprayed into a high‐resolution mass spectrometer to determine their base compositions. The combined base compositions from multiple PCR reactions (multiple primer sets) support the identification of microorganisms in a sample. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com]