Figure 2.

Evidence of parasite‐specific deubiquitinating and deNeddylating activity. Parasite and uninfected red blood cell (RBC) lysates were reacted with (A) HA‐Ub‐VME or (B) FLAG‐Nedd8‐VS followed by SDS‐PAGE and immunoblot with anti‐HA‐HRP or anti‐FLAG‐HRP where appropriate. Probe alone and lysate alone are shown in the left three lanes of each panel. Reaction profiles in the presence or absence of NEM are shown in the right four lanes of each panel. Numbers on the left‐hand side of (A) indicate molecular weights in kDa. The reactive polypeptide later excised for mass spectrometric analysis is indicated by the arrow. Pf, Plasmodium falciparum lysate.