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. 2014 Mar 5;58(3):180–187. doi: 10.1111/1348-0421.12127

Figure 1.

Figure 1

Effects of glycycoumarin, glycyrin and glycyrol isolated from G. uralensis on HCV RNA replication and protein synthesis. (a) Huh 7.5 cells infected with HCV J6/JFH1 and treated with either glycycoumarin (20 μg/mL), glycyrin (15 μg/mL), glycyrol (10 μg/mL) or left untreated were subjected to western blot analysis using monoclonal antibody against the HCV NS3 protein at 1 and 2 days post‐infection. GAPDH served as an internal control to verify equal amounts of sample loading. Signal intensities of NS3 were normalized to the corresponding GAPDH signal. (b) Amounts of HCV RNA in the cells described in (a) were measured by real‐time quantitative RT‐PCR analysis. These amounts were normalized to GAPDH mRNA expression. Data represent means ± SEM of data from two independent experiments. The value for the untreated control at 1 day post‐infection is arbitrarily expressed as 1.0. * P < 0.001, compared with the control. (c) Amounts of HCV infectious particles in the supernatants of the cultures described in (a) and (b) were determined: data for glycycoumarin, glycyrin, glycyrol and the untreated control are shown. Data represent means ± SEM of data from two independent experiments. * P < 0.05; † P < 0.01, compared with the untreated control; dpi, days post infection.