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. 2012 Jul 26;56(8):554–561. doi: 10.1111/j.1348-0421.2012.00467.x

Figure 2.

Figure 2

Immunogenicity of fusion protein rS450–650‐CRT vs rS450–650 and a mixture of rS450–650 + rCRT/39–272. Serum samples from BALB/c mice (5/group) immunized s.c. 14 days earlier with rCRT/39–272, rS450–650, rS450–650‐CRT or an equal proportion mixture rS450–650 plus rCRT/39–272 were serially diluted and analyzed, in triplicate wells, using rS450–650‐based ELISAs. The detection Abs were goat‐anti‐mouse IgG with OPD as substrate, and the results expressed as mean absorbance at 492 nm±SD.**P < 0.01; ***P < 0.001; ns, not significant.