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. 2012 Jul 26;56(8):554–561. doi: 10.1111/j.1348-0421.2012.00467.x

Figure 4.

Figure 4

Induction of S450–650‐specific IgG production in BALB/c‐nu mice by vaccination with rS450–650‐CRT. Groups of BALB/c‐nu mice were s.c. immunized at the base of the tail with rCRT/39–272, rS450–650 or rS450–650/CRT (30 μg/mouse, in 50 μL PBS). Serum samples, collected 21 days later, were assayed for anti‐S450–650 IgG using ELISA. The detection Abs were HRP‐labeled goat‐anti‐mouse IgG with OPD as substrate and the results are expressed as mean absorbance at 492 nm ± SD. **P < 0.01; ***P < 0.001; ns, not significant.