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rCRT/39–272 and rS450–650‐CRT induce maturation of dendritic cells in vitro. Bone‐marrow‐ derived mouse immature DCs were stimulated with, or without, LPS (10 μg/mL), rCRT/39–272 (30 μg/mL), rS450–650‐CRT (30 μg/mL) or rEGFP (30 μg/mL) for 24 hrs in R10 medium at 37 °C with 5% CO2. The cells were then double stained with FITC‐conjugated anti‐CD40 mAb and PE‐labeled mAb against CD11c.
