Table 1.
Characterization of 10 microsatellite loci isolated from Miniopterus magnater
| Locus | Primer sequence (5′–3′) | Fluorescent dye | Allele size range (bp) | Repeat motif | No. of alleles | T a (˚C) | H E | H O | GenBank Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CH37* | F: GAAATCAGATGGAAATGCCTCA | FAM | 100–146 | (TG)3 CG(TG)12 | 18 | 62 | 0.912 | 0.487 | EU010361 |
| R: CTCCAGCCACATGGTAATGC | |||||||||
| CHD2 | F: GGAGGCTGATAAGTTGGTCTA | FAM | 207–231 | (AC)24 | 13 | 56 | 0.900 | 0.822 | EU010362 |
| R: GCACTTGGCAATGGATGTA | |||||||||
| CHB6* | F: CATGGCTTTGTGTCTCTGTCC | HEX | 373–389 | (AC)9 | 9 | 56 | 0.846 | 0.447 | EU010363 |
| R: TGTTATCTGCTATGCGACCCT | |||||||||
| CH87 | F: ATGGCACCCAGTAGGTTTCC | FAM | 140–152 | (TG)6 CG(TG)6 (CG)3(TG)3 TA(TG)3 | 6 | 59 | 0.676 | 0.660 | EU010364 |
| R: TTCCCACAGAGCCTGCCTGT | |||||||||
| CHB12 | F: GGGGATGTGAGATATGAGT | FAM | 244–262 | (TG)11 | 10 | 54 | 0.780 | 0.792 | EU010365 |
| R: ACAGCTCATCATGTTATAGC | |||||||||
| CHD3 | F: CCAGTGGCAACTTTCTTGTT | HEX | 290–314 | (TG)14 | 10 | 62 | 0.732 | 0.558 | EU010366 |
| R: AGCCCAGTTTGTCACATCTC | |||||||||
| CHA14 | F: ATGAATGGCAGGTAGGTGAC | TAMARA | 237–283 | (TG)19 | 15 | 58 | 0.733 | 0.625 | EU010368 |
| R: CTTGCTACTTAGGCTTTCTCC | |||||||||
| CH13 | F: GGGAAAGAATAAAAGAGGAGC | FAM | 166–230 | (TG)15 | 27 | 58 | 0.951 | 0.957 | EU010369 |
| R: ATTCGCAGTTTCACTATGGC | |||||||||
| CH42* | F: TGCTTCCTAGCATCCCTCT | HEX | 272–294 | (TG)11 | 12 | 58 | 0.845 | 0.489 | EU010371 |
| R: CCATTCAACGCACATAAAC | |||||||||
| CH85* | F: GCTGGGTTTGTGCCAAGGAC | TAMARA | 328–370 | (AC)17 | 16 | 63 | 0.880 | 0.364 | EU010372 |
| R: GATGTAAAGCGGGGCGTGAA |
Abbreviations used: F, forward primer, 5′‐labelled (FAM, TAMARA or HEX); R, reverse primer; T a, annealing temperature of the primer pairs; H O, observed heterozygosity; H E, expected heterozygosity; *indicates significant deviation from Hardy–Weinberg equilibrium after Bonferroni correction for multiple comparisons (adjusted α = 0.005).