Figure 1.

Schematic diagram of the genome organization of KOR/KNU‐1811/2018/G1‐PLCP. (a) The Korean KNU‐1811 genome contains a single open reading frame (ORF) flanked by a long 5′ untranslated region (UTR) (813‐nt) and a short 3′ UTR (71‐nt), followed by a poly (A) tail (An). The stem‐loop secondary structure on the 5′ UTR represents an internal ribosome entry site (IRES). The torovirus (ToV) papain‐like cysteine protease (PLCP) gene is presented as a blue box that is inserted at the 2C/3A cleavage junction. The 5′‐ and 3′‐boundary sequences of enterovirus (EV‐G) 3C protease (3C^pro) cleavage sites are shown in an enlarged blue box. Vertical lines indicate the polyprotein processing sites creating precursor polyproteins P1, P2, and P3 by 3C^pro. (b) Multiple alignment of the amino acid sequences of the PLCP regions of the recombinant EV‐G and ToV strains [Colour figure can be viewed at http://wileyonlinelibrary.com]