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. 2019 Sep 5;67(1):11–17. doi: 10.1111/tbed.13333

Figure 1.

Figure 1

Location, genomic and evolutionary characteristics of the novel phlebovirus found in a Costa Rican sloth. Geographic distribution of ANHV‐related viruses, that is, ANHV from Brazil and Penshurt virus (PEHV) from Costa Rica. Map built with Quantum GIS—QGIS (http://qgis.osgeo.org) (a). Maximum likelihood phylogenetic tree based on translated amino acid sequences of the L, M, N and NSs genes with highlighted trees showing viruses closely related to ANHV and PEHV. Black circles at nodes represent support values of ≥0.75 from 1000 bootstrap replicates (b). Genome organization of the new PEHV found in Costa Rican sloths, showing genomic segments and encoded proteins (RdRp on L; NSm, Gn and Gc on M; nucleocapsid (N) and non‐structural (NSs) on S segment). Proteins were predicted according to BLASTp analysis comparing the PEHV genome with those from other pheboviruses. Red, non‐synonymous substitutions compared to ANHV reference sequences (c). Mean dN/dS (ω) ratios on L, M, N and NSs genes using different methods as indicated below (d). Pressure analyses included the two ANHV‐related strains ANHV and PEHV and Bujaru, Candiru, Chagres, CoAr, Munguba, Punta Toro, Rift Valley, Salobo, Turuna and Uriana virus (d). Accession number for all phlebovirus reference sequences (segments L, M and S) used: Adana (KJ939330, NC_029128, NC_029129), Anhanga (NC_033836, NC_033846, NC_033837), Armero (HM566140, HM566141, HQ661807) Bujaru (KX611388, KX611389, KX611390), Buenaventura (KP272001, KP272002, EF201839), Candiru (NC_015374, NC_015373, NC_015375), Chagres (HM566147, HM566146, HM566148), CoAr (HM566152, HM566154, HM566153), Jacunda (HM466934, HM466935, HM466936), Karimabad (KF297912, KF297913, KF297914), Munguba (HM566164, NC_033847, NC_033831), Punta Toro (KP272004, KP272005, KP272006), Rift Valley (DQ375409, DQ380204, DQ380146), Salobo (HM627185, HM627183, HM627184), Sandfly Naples (HM566172, HM566169, HM566170), Sandfly Sicilian (NC_015412, NC_015411, NC_015413), Palma (NC_021242, NC_021243, NC_021244), Toros (KP966619, NC_037614, NC_037615), Turuna (HM119431, HM119432, HM119433), Uriurana (HM566189, HM566188, HM566190), Urucuri (NC_033841, NC_033842, NC_033843) [Colour figure can be viewed at http://www.wileyonlinelibrary.com/]