Table 1.
Primers for detection and quantification of PEHVa
| Use | Gene | Name | Oligonucleotide sequence |
|---|---|---|---|
| RT‐PCR | L | L69F | CTTAGCTATGTTGAAACCCTATACA |
| L409R | GCCAATAGGGCAGATTTCTCTG | ||
| L394R | TCTCTGCTCCTCTAGTTGTGG | ||
| qRT‐PCR | L | PEHV‐rtF | AAGATTGTGAGAATGTGCACTGAGT |
| PEHV‐rtR | TTAGTGTCCGCTCCCTTTCCT | ||
| PEHV‐rtP | TTGGAACAGTATTCTCACACTTGCCGGCTb |
Thermocycling involved reverse transcription at 55°C for 20 min followed by 94°C for 3 min and then 45 cycles of 94°C for 15 s and 58°C for 30 s. PCR was done using the One‐Step SuperScript III RT‐PCR kit for first‐round and real‐time RT‐PCR reactions and the Platinum Taq PCR kit (Thermo Fisher) for second round reactions.
Labelled with fluorescein amidite (FAM) at the 5′ end and a dark quencher at the 3′ end.
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