Table 1.
Oligonucleotide primers used for amplification of the complete genome of PEDV HLJBY by reverse transcription–polymerase chain reaction
| Sequence (5ꞌ−3ꞌ) | Nucleotide position | Product size（bp） | |
|---|---|---|---|
| 1F | ACTTAAAGAGATTTTCTATCTACGGATA | 1 | 6,146 |
| 1R | AAACACTGTAATTAAATTACGTGACAT | 6,146 | |
| 2F | ATACTGTTTTTGATCATGGCACTGGTA | 5,934 | 4,130 |
| 2R | AGAATTTGCTTTCCACCAAAATTCTTA | 10,066 | |
| 3F | TAATGGTATGACAACAGTGGGCAATAC | 9,964 | 6,505 |
| 3R | AATTAGTGCACATAGAGACCTCATCCA | 16,469 | |
| 4F | GCTCAGTACCTTTTCTCCACTGTCAAT | 16,408 | 4,123 |
| 4R | GCACTAGCAACTTACCATTCTTCAACA | 20,531 | |
| 5F | GCATAAGGCTACAGTTGTTGTTAATTT | 20,469 | 4,630 |
| 5R | ATAATAAGCTGCTTTACCATTGAGAAA | 25,099 | |
| 6F | TTGCACTGTTTAGAGCGTCTTCTTTGA | 24,927 | 3,000 |
| 6R | GTGTATCCATATCAACACCGTCAGGTC | 27,927 |
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