Table 4.
Correlation between slime production ability using CRA assay and distribution of biofilm-associated genes, MSCRAMMs genes, and regulatory genes in S. aureus isolates.
| Criteria | Sheep Mastitis Isolates (n = 28) | Human Isolates (n = 19) | Food Isolates (n = 8) | |||||
|---|---|---|---|---|---|---|---|---|
| Slime Producing (18) | No Slime Producing (10) | Slime Producing (13) | No Slime Producing (6) | Slime Producing (8) | No Slime Producing (0) | |||
| Polysaccharide intercellular adhesin and biofilm-associated protein genes | icaACD | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | |
| bap | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Genes encoding microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) | bbp | 17 (94.4%) | 10 (100%) | 12 (92.3%) | 6 (100%) | 6 (75.0%) | 0 | |
| clfA/B | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||
| cna | 4 (22.2%) | 2 (20.0%) | 9 (69.2%) | 4 (66.6%) | 4 (50.0%) | 0 | ||
| ebh | 18 (100%) | 10 (100%) | 9 (69.2%) | 3 (50.0%) | 8 (100%) | 0 | ||
| ebpS | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||
| eno | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||
| fib | 18 (100%) | 10 (100%) | 6 (46.1%9 | 2 (33.3%) | 8 (100%) | 0 | ||
| fnbA | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||
| fnbB | 18 (100%) | 2 (20.0%) | 8 (61.5%) | 2 (33.3%) | 8 (100%) | 0 | ||
| map | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||
| sasG | 6 (33.3%) | 3 (30.0%) | 10 (76.9%) | 4 (66.6%) | 8 (100%) | 0 | ||
| sdrC | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||
| sdrD | 13 (72.2%) | 6 (60.0%) | 12 (92.3%) | 4 (66.6%) | 7 (87.5%) | 0 | ||
| vwb | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||
| Regulatory genes | agr | I | 5 (27.7%) | 2 (20.0%) | 7 (53.8%) | 5 (83.3%) | 2 (25%) | 0 |
| II | 1 (5.5%) | 2 (20.0%) | 3 (23.0%) | 1 (16.6%) | 3 (37.5%) | 0 | ||
| III | 12 (66.6%) | 6 (60%) | 3 (23.0%) | 0 | 3 (37.5%) | 0 | ||
| IV | 0 | 0 | 0 | 0 | 0 | 0 | ||
| sarA | 18 (100%) | 10 (100%) | 13 (100%) | 6 (100%) | 8 (100%) | 0 | ||