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. 2020 Jan 28;9(2):84. doi: 10.3390/pathogens9020084

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Time-kill curve for C. albicans SC 5314 (A) and C. glabrata ATCC 2001 (B) in the presence and absence of the Platonia insignis hydroethanolic extract and fractions. After different treatment times (0, 3, 6, 9, 12, 24, 36, and 48 h) at 37 °C for PiHE, DCMF, EAF (MIC and 2 × MIC), and AMB (MIC), 20 μL samples were withdrawn and subjected to serial dilutions before seeding on the Sabouraud Dextrose Agar (SDA) for counting colony-forming units (CFU)/mL. Data are presented as means of three biological replicates ± standard deviation and were analyzed at each time in comparison to the control samples. Symbols represent the quantification of colony-forming units grown at each tested time evaluated for each treatment.