Figure 6.

Effect of the P. insignis extract and fractions on C. albicans SC 5314 and C. glabrata ATCC 2001 biofilm formation and mature biofilm inhibition. Biofilm formation and mature biofilms were analyzed by measuring the absorbance at 590 nm (A–D) and percent viability (E–H). Metabolic activity was evaluated by the MTT reduction assay. Results were compared to the vehicle control group (1% DMSO), positive control group (amphotericin ½ MIC, ¼ MIC, 2 × MIC, 4 × MIC), and negative control (PBS). Data are represented as the mean ± standard deviation for three independent experiments with a significance of p < 0.05, and were analyzed by ANOVA, followed by Tukey’s post-test. * p < 0.05.