Figure 9.
Effect of the P. insignis extract and fractions on RAW 264.7 cell viability (A–C) and on the hemolytic activity of sheep erythrocytes (D). Cells at the amount of 1 × 106/mL were used in assays. The effects of PiHE, DCMF, and EAF on RAW 264.7 cells are shown, and viability above 70% was considered non-cytotoxic according to the ISO 10993-5 (2009) when compared to the untreated control (A–C). The percent hemolytic activity was compared to Triton X-100, as the hemolysis control (D). Triton X hemolytic activity was considered as 100% for data analysis. Data were analyzed by ANOVA with Tukey’s post-test, with * p < 0.05.