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. 2019 Dec 25;9(1):22. doi: 10.3390/pathogens9010022

Figure 9.

Figure 9

PCR detection of integrated and excised forms of: (a) ICEs of strain NSUI084: whole tandem structure or Tn1549 (ICE1) and Tn5252 (ICE2) separate elements; (b) ICE_SsuNSUI086_rplL. The sizes of the PCR fragments obtained for the amplification of controls attL (left site of the integrated element) and attR (right site of the integrated element), attB (empty site) and attI (circular form) were confirmed by parallel migration of a DNA ladder. The primers pairs used for these amplifications are listed in Table S2.