Figure 2.

Berberine induced apoptosis of MGC803 cells. (a) Berberine treatment at 10, 20, and 40 μm for 24 h promoted the apoptosis of MGC803 cells, as detected by flow cytometry and statistically analyzed in (b). (c) Berberine treatment at 10, 20, and 40 μm for 24 h downregulated the protein expression of antiapoptosis protein Bcl-2 and promoted the protein expression of proapoptosis protein Bax and activated the expression of caspase3 protein. (d) The protein expression of apoptosis-related proteins of MGC803 cells after berberine treatment was quantified. ^∗∗∗∗P < 0.0001, ^∗∗∗P < 0.001, ^∗∗P < 0.01, and ^∗P < 0.05 vs controls.