Figure 1.

Seed-based connectivity varies based on the anesthetic used. (a) Representative seed-based connectivity maps for Avertin, ketamine–xylazine, urethane, chloral hydrate, and isoflurane. (b) Averaged connection matrices among the eight seeds. The absolute value of the coefficient of variation of the correlation coefficients for each pair in the matrix (|CV|) is shown in the upper right of each panel. (c) Median |CV| for each of the anesthetics tested (error bars are 25–75% interquartile range). (d) Average correlation coefficient of the map for all positive values associated with each seed. The number of mice for each experiment was n = 5 for Avertin, n = 5 for ketamine–xylazine, n = 5 for urethane, n = 5 for chloral hydrate, and n = 4 for isoflurane. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Bonferroni-corrected for parametric tests and corrected using Dunn's statistical hypothesis testing for non-parametric tests.