Figure 3.

Maclpil modulates migration and phagocytosis by its actin-relevant neighbor gene LCP1. (a) RT-PCR results show that when Maclpil was knocked down by siRNA in cultured M(LPS), both Maclpil and LCP1 expressions were inhibited for 48 h after LPS addition. ****, p < 0.0001, one-way ANONVA, n = 3. (b) In vitro transwell cell migration assay. The knockdown of LCP1 or Maclpil decreased the cell migration from the upper chamber to the lower chamber. Mock control (cells with transfection reagent only, without adding siRNA). (c) In vitro cell phagocytosis assay. FACS results showed that when LCP1 or Maclpil was knocked down by siRNA, the numbers of cells phagocytosed FITC⁺ latex beads were significantly reduced. Mock control (cells with transfection reagent only, without adding FITC⁺ beads). *, ***, p < 0.05, p < 0.001, one-way ANOVA.