Table 1.
SEQUEST search results LC-MS/MS analysis of DENV-3 binding protein on SH-SY5Y cell membrane
| Accession | Gene symbol | Description | Sequest Score | PSMs | Unique Peptides | MW [kDa] |
|---|---|---|---|---|---|---|
| A | ||||||
| 62414289 | VIM | vimentin | 1.85 | 1 | 1 | 53.62 |
| 32189394 | ATP5B | ATP synthase subunit beta, mitochondrial precursor | 2.78 | 1 | 1 | 56.53 |
| 4501881 | ACTA1 | actin, alpha skeletal muscle | 1.64 | 1 | 1 | 42.02 |
| B | ||||||
| 221307584 | PHB2 | prohibitin-2 isoform 1 | 25.53 | 9 | 8 | 33.276 |
| 5174447 | GNB2L1 | guanine nucleotide-binding protein subunit beta-2-like 1 | 21.85 | 8 | 5 | 35.055 |
| 4506663 | RPL8 | 60S ribosomal protein L8 | 18.08 | 5 | 4 | 28.007 |
| 4507879 | VDAC1 | voltage-dependent anion-selective channel protein 1 | 17.83 | 7 | 4 | 30.754 |
| 17158044 | RPS6 | 40S ribosomal protein S6 | 15.62 | 5 | 5 | 28.663 |
| 386869503 | RPS3 | 40S ribosomal protein S3 isoform 2 | 14.90 | 7 | 3 | 28.468 |
| 4506661 | RPL7A | 60S ribosomal protein L7a | 14.40 | 5 | 5 | 29.977 |
| 208879465 | VDAC3 | voltage-dependent anion-selective channel protein 3 isoform 2 | 8.02 | 3 | 1 | 30.77 |
| 4502709 | CDK1 | cyclin-dependent kinase 1 isoform 1 | 7.73 | 3 | 3 | 34.074 |
| 15431301 | RPL7 | 60S ribosomal protein L7 | 6.28 | 2 | 2 | 29.207 |
| C | ||||||
| 156071459 | SLC25A5 | ADP/ATP translocase 2 | 282.03 | 107 | 15 | 32.83 |
| 4506725 | RPS4X | 40S ribosomal protein S4, X isoform X isoform | 210.99 | 89 | 28 | 29.58 |
| 55749577 | SLC25A4 | ADP/ATP translocase 1 | 158.48 | 63 | 4 | 33.04 |
| 528281407 | PHB | prohibitin isoform 1 | 85.93 | 33 | 15 | 29.79 |
| 208973244 | YWHAZ | 14–3-3 protein zeta/delta | 77.24 | 32 | 14 | 27.73 |
| 4505753 | PGAM1 | phosphoglycerate mutase 1 isoform 1 | 61.48 | 20 | 15 | 28.79 |
| 316659409 | ACTG1 | actin, cytoplasmic 2 | 55.26 | 24 | 10 | 41.77 |
| 4507949 | YWHAB | 14–3-3 protein beta/alpha | 46.59 | 19 | 5 | 28.07 |
| 5803227 | YWHAQ | 14–3-3 protein theta | 43.68 | 20 | 9 | 27.75 |
| 21464101 | YWHAG | 14–3-3 protein gamma | 38.78 | 17 | 8 | 28.29 |
Proteins are arranged in descending order based on scores obtained (A) ~ 50 kDa protein (B) ~ 31 kDa protein (C) ~ 28 kDa. VIM, 53 kDa proteins, PHB2, 33 kDa protein and PHB2, 30 kDa proteins were identified as bona fide protein to serve as a DENV-3 receptor on SH-SY5Y cells. Bold fonts represent the proteins identified in this study for further characterization based on the results obtained in the algorithm