Fig. 3.

Effect of MCPyV LT on early and late promoter activity in MCC13 cells. Cells were co-transfected with 1 μg luciferase reporter plasmid with the early promoter of the MCPyV variants and 100 ng of empty pcDNA3.1 vector (EV) or LT expression plasmid (a and c), or with 500 ng of empty vector or LT expression plasmid (b and d). The effect on the early MCPyV promoters is depicted in A and B, while the effect on the late promoters is shown in C and D. Luciferase activity was corrected for protein concentration in each sample, and the activity in the presence of an empty vector was arbitrary set as 100%. Each bar represents the average of three independent parallels + SD. Similar results were obtained in an independent experiment; *p < 0.05; **p < 0.01