Figure 2.

Overview of the CRISPR-Cas12a system to directly target plasma for the detection of EGFR mutations. Plasma or supernatant of cultured cells were processed for simply PCR amplification. The PCR product was a mixture with LbCas12a, crRNA, and ssDNA FQ reporter. The reaction was proceeded for up to two hours at room temperature on a plate reader for fluorescent readout.