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Sequences of guide RNA and reporter substrates.
| Guide RNA’s Name | Sequence (5′->3′) |
|---|---|
| LbCas12a crRNA-EGFR790-C | UAAUUUCUACUAAGUGUAGAUAUCACGCAGCUCAUGCC |
| LbCas12a crRNA-EGFR790-T | UAAUUUCUACUAAGUGUAGAUAUCAUGCAGCUCAUGCC |
| LbCas12a crRNA-EGFR858-T | UAAUUUCUACUAAGUGUAGAUGGCUGGCCAAACUGCUG |
| LbCas12a crRNA-EGFR858-G | UAAUUUCUACUAAGUGUAGAUGGCGGGCCAAACUGCUG |
| Substrate’s name | Sequence (5′->3′) |
| ssDNA-FQ reporter | /56-FAM/TTATT/3IABKFQ/ |
