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PHGDH coupled biochemical assay system. (A) Schematic representation of the coupled system. Continuous measurement of PHGDH activity for initial PHGDH assay (B), for PHGDH/PSAT1 coupled assay (C), and for present fully coupled assay (D). (3-PG, 3-phosphoglycerate; 3-PPyr, 3-phosphopyruvate; 3-PSer, 3-phosphoserine).
