Fig. 3.

HMGB1 impaired the therapeutic effects of MSCs on renal IRI in mice. Mice were subjected to 30 min bilateral renal IRI and then received differently treated-MSCs (1 × 10⁶) via the tail vein 2 h after reperfusion. Mice were sacrificed at day 1 to collect blood and kidney tissues. a Serum levels of Scr and BUN were detected as kidney function. b Representative images of H&E staining are exhibited and ATN scores were calculated. c Representative photographs of TUNEL staining are demonstrated and quantitative analysis of TUNEL-positive cells was performed. Data were shown as mean ± SD (n = 6 mice per group). *P < 0.05