Fig. 5.

HMGB1 inhibited iNOS mRNA expression via TLR4. a MSCs were treated with HMGB1, A box or B box at indicated concentrations for 24 h in the presence or absence of IFN-γ and TNF-α (5 ng/ml). mRNA level of iNOS was analyzed by qPCR. b MSCs were treated with IFN-γ and TNF-α at graded concentrations for 24 h in the presence or absence of HMGB1 (1 μg/ml). mRNA level of iNOS was analyzed by qPCR. c MSCs were treated with IFN-γ and TNF-α (5 ng/ml) in the presence or absence of HMGB1 (1 μg/ml). mRNA level of iNOS was analyzed by qPCR at indicated time point. d MSCs were treated with HMGB1 at indicated concentrations for 24 h in the presence or absence of IFN-γ and TNF-α (5 ng/ml). mRNA level of chemokines was analyzed by qPCR. e Receptors were determined by western blot. MSCs were treated with HMGB1 in the presence or absence of IFN-γ and TNF-α. RAGE or TLR4 siRNA were administrated, and iNOS mRNA f was examined. Each data point represented the average value of triplicated wells from each independent experiment. Data were pooled from three independent experiments. Data were shown as mean ± SD. *P < 0.05, **P < 0.01