Figure 1.

Functional analysis of T‐and B‐cells in stem cells products. Proliferation of T‐and B‐cells in donor stem cell products was analysed using the FASCIA method. B‐cell proliferation was only measured upon stimulation with PWM and EBV, depicted PMW (b) and EBV (b) in 1a. Cells were unstimulated or stimulated with PWM, SEA/SEB, CMV, VZV, HSV, EBV, tetanus toxoid, candida, influenza antigen or adenovirus antigen. Proliferation was expressed as stimulation indices (SI) with an SI > 4 considered positive. A: Proliferation against antigens, horizontal line represents mean SI and the dotted line represents cut‐off. B: Stem cell products were divided into two groups, ‘infection’ (I, n = 12) and ‘no infection’ (NI, n = 9) based on patients’ clinical outcome. A composite analysis was performed where a positive response against any of HSV, EBV, CMV, VZV, candida or adenovirus rendered 1 point with a maximum of 6 points. **P‐value < 0·01.