Table 2.
Viral inactivation during pasteurization (10 h at 60 °C) of Fraction V products
| Time at 60 °C (h) | 5% Albumin | 25% Albumin | HPPS | |||
|---|---|---|---|---|---|---|
| WNV a | BVDV b | WNV a | BVDV b | WNV a | VV a | |
| Unheated a | 7·4 | 4·8 | 6·7 | 4·4 | 7·4 | 6·0 |
| 0·5 | 0·8 | ≤ 0·7 | ≤ 0·3 | ≤ 0·7 | ≤ 0·3 | 1·7 |
| 1 | 0·2 | ≤ 0·7 | ≤ 0·3 | ≤ 0·7 | ≤ 0·3 | 0·8 |
| 2 | ≤ 0·3 | ≤ 0·7 | ≤ 0·3 | ≤ 0·7 | ≤ 0·3 | ≤ 0·3 |
| 5 | ≤ 0·3 | ≤ 0·7 | ≤ 0·3 | ≤ 0·7 | ≤ 0·3 | ≤ 0·3 |
| 10 | ≤ 0·0 d | ≤ 0·7 | ≤ 0·0 d | ≤ 0·7 | ≤ 0·3 | ≤ 0·3 |
| Log10 reduction factor | 7·4 | 4·1 | 6·7 | 3·7 | 7·1 | 5·7 |
a
Data from the West Nile virus (WNV) and vaccinia virus (VV) samples are expressed as log10 plaque‐forming units/ml.
b
Data from the bovine viral diarrhoea virus (BVDV) samples are expressed as log10 tissue culture infectious dose 50/ml.
c
Virus was spiked into samples once they reached 60 °C. As the potential existed for immediate inactivation, the initial virus concentration was determined from an unheated sample.
d
Increased volume testing. WNV values represent the mean of three determinations, BVDV values represent the mean of two (albumin) or four [human plasma protein solution (HPPS)] determinations and VV values represent a single determination.