Figure 4.

The effects of time and opsonization on Matrix Metalloproteinase 9 (MMP-9) release (data from Figure 2D) were re-analyzed by a two-way ANOVA with Bonferroni post-hoc analysis (A). Gelatin zymography of supernatants from neutrophils challenged with non-opsonized or opsonized M. smegmatis was performed on a 9% SDS-PAGE gel and stained with Coommassie Blue (B). The different isoforms of MMP-9 are indicated. Enzymatic activity was measured by quantifying the intensity of the clear bands where digestion took place (C). Data are graphed as the mean ± SEM of band intensity from four independent experiments. A two-way ANOVA of time and opsonization on MMP-9 release with Bonferroni post-hoc testing was conducted on this data.