Skip to main content
. 2020 Feb 15;9(2):123. doi: 10.3390/pathogens9020123

Figure 6.

Figure 6

Neutrophils were unstimulated (basal), stimulated with Peptoanaerobacter stomatis (MOI 10), or different treatments of M. smegmatis (MOI 6) for 1, 2, or 6 h. Exocytosis of secretory vesicles was measured by flow cytometry as the surface membrane expression of CD35 (A). Gelatinase granule exocytosis was measured as the presence of MMP-9 in the supernatants of neutrophils by ELISA (B). Specific granule exocytosis was measured by flow cytometry as the surface membrane expression of CD66b (C) and the presence of Lactoferrin in the neutrophil supernatants by ELISA (D). Azurophilic granule release was measured as the presence of Myeloperoxidase (E) and Elastase (F) in the supernatants of neutrophils. The histograms in A and C show the flow cytometry data as the average mean channel fluorescence (mcf) ± SEM of three independent experiments. B, D, E, and F show the average release of each granule marker ± SEM from five independent experiments. * = p value < 0.05, ** = p value < 0.01, *** = p value < 0.001.