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Knockout and complementary strategy of the CgSCD1 gene, and electrophoretic identification of the ΔCgscd1 and Cgscd1com strains. (A) Diagram describing the homologous recombination targeted gene deletion of CgSCD1. (B) Plasmid pAg1-H3-scd1-com was used for Agrobacterium transformation to obtain the complementation mutant Cgscd1com strain. (C) Confirmation of the deletion mutant ΔCgscd1. (D) Confirmation of the complementation mutant Cgscd1com.
