Figure 3.

Phenotypic characteristics of the wild-type (WT), ΔCgscd1, and Cgscd1^com strains of C. gloeosporioides. (A) Colonies grown in potato dextrose agar (PDA) for 7 days, at 25 °C (images on the two columns on the left). Germinated conidia, from cultures grown for 7 days, at 25 °C (images on the two columns on the right). Arrows point to mature appressoria. Ap, appressoria; C, conidia. Scale bar (for (A)) = 10 µm. (B) Periodic (24 h) measurement of colony growth diameter. (C) Dry weight of mycelia grown on cellophane-covered PDA for 7 days. (D) Germination and appressorium formation of each strain. (E) Quantitative differences in the WT, ΔCgscd1, and Cgscd1^com strains in lateral germination events of appressoria (1, 2, 3 represent the number of appressoria produced by a single conidium; n = 100). For (C,E): p < 0.05 (*).