Table 3.

List of antibodies.

Primary antibodies

RAC1 (#610651, BD Transduction)

CDC42 (#2462, Cell Signaling)

RHOA (#ARH03, Cytoskeleton)

RHOC (#3430S, Cell Signaling)

RHOGDI (#2564S, Cell Signaling)

p44/42 MAPK (ERK1/2) (#9102, Cell Signaling Technologies)

phospho-p44/42 MAPK (T202/Y204) (#9101, Cell Signaling Technologies)

MEK1 (#2352, Cell Signaling Technologies)

phospho-MEK1 (Ser217) (#9154, Cell Signaling Technologies)

phospho-MEK1 (Ser298) (#98195, Cell Signaling Technologies)

β-actin (6221, BioLegend; A1978, Sigma)

α-tubulin (12G10, DSHB)

CUL3 (#2759, Cell Signaling Technologies)

NF1 (#14623, Cell Signaling Technologies)

Cleaved Caspase 3 (Asp175) (#9661, Cell Signaling Technologies)

Secondary antibodies

Goat anti-rabbit IRDye 680 RD (#925-68071, LI-COR)

Donkey anti-mouse IRDye 800 CW (#610-731-124, Rockland)

Goat anti-mouse Alexa Fluor 680 (#A21058, Invitrogen)

Goat anti-rabbit Alexa Fluor 790 (#A11369, Invitrogen)