Figure 3.

(A) Composite photograph of plasma separation on cartridge. (B) Hemoglobin levels in the plasma obtained from biochemical or centrifugation separation methods are not statistically different as measured by paired t-test. This study demonstrates that biochemical plasma separation does not induce excessive stress on the red blood cells (RBCs). (C) Whole blood from a single donor was lysed using either standard osmotic methods on the bench (left) or via osmotic lysis on the digital microfluidic (DMF) cartridge (right). The scatter at 775 nm is comparable in both samples, indicating that the DMF lysis method is as effective as the standard bench osmotic shock technique. (D) Sample input volume (x-axis) versus impedance (y-axis); circle: fail, triangle: pass. AU: arbitrary units.