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. 2020 Apr 1;45(6):1783–1792. doi: 10.3892/ijmm.2020.4562

Figure 1.

Figure 1

Knockdown of CD44 inhibits cell growth of H460 cells and enhances cisplatin sensitivity. (A) Cisplatin cytotoxicity of H460 for 48 h was tested by a CCK-8 assay. (B) sictr and siCD44 were transfected into H460 cells followed by cisplatin treatment at concentrations of 2.5, 5 and 10 µM for 48 h. Cell viability of H460 was detected by CCK-8 assay. (C) After transfection with sictr and siCD44 respectively, H460 cells were treated with or without cisplatin (10 µM) and proliferative curves of H460 cells were assessed at 0, 1, 2, 3 and 4 days post treatment using CCK-8. (D) The knockdown efficiency of CD44 in H460 was evaluated using reverse transcription-quantitative PCR and western blotting. Data are displayed as mean ± SD of 3 independent experiments. *P<0.05 vs. sictr; #P<0.05 vs. sictr + cisplatin. CCK-8, Cell Counting Kit-8; sictr, control siRNA; siCD44, CD44 siRNA.