Figure 2.

Effects of siCD44, cisplatin or their combination on cell cycle distribution of H460 cells. H460 cells were seeded into 6-well plate at 1.5×10⁵ cells/well and transfected with sictr and siCD44 respectively for 24 h, followed by treatment of cisplatin at concentration of 10 µM for another 24 h. (A) Transfected cells were stained with PI for cell cycle analysis by flow cytometry. Cell distribution was analyzed by MFLT32. (B) Corresponding statistical analysis of cell distribution. (C) The protein expressions of CD44, c-Myc, CDK2, CDK4, CDK6 and cyclin D1 in H460 were analyzed by western blotting, with GAPDH as loading control. Data were presented as mean ± SD of 3 independent experiments. ^*P<0.05 vs. sictr; ^#P<0.05 vs. sictr + cisplatin. sictr, control siRNA; siCD44, CD44 siRNA; PI, propidium iodide.